Saint Theodota was a young widow with three children, whom she raised in piety. The Great Martyr St Anastasia lived with her in Macedonia, and the two women visited Christians in prison and took care of them.

Arrested as a Christian, Theodota was sent to Nicetas, the governor of Bithynia, for interrogation. Since she refused to deny Christ, she and her three children were sentenced to death, beaten, and thrown into a fiery furnace.